PrimePCR™ Probe Assay: MAML1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MAML1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAML1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053187
Assay Design:   exonic
Chromosome Location:   5:179203061-179203170question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000292599

Gene Information

This protein is the human homolog of mastermind a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway. [provided by RefSeq Jul 2008]

Gene Symbol:   MAML1
Gene Name:   mastermind-like 1 (Drosophila)
Aliases:   FLJ53540, KIAA0200, Mam-1, Mam1
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000161021
Entrez:   9794
Chromosome Mapping:   5q35

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