PrimePCR™ Probe Assay: FCRLB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FCRLB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: FCRLB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053270
Assay Design:   exonic
Chromosome Location:   1:161697270-161697415question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000367948 ENST00000367946 ENST00000367945 ENST00000336830 ENST00000367944 ENST00000392158

Gene Information

FCRL2 belongs to the Fc receptor family. Fc receptors are involved in phagocytosis antibody-dependent cell cytotoxicity immediate hypersensitivity and transcytosis of immunoglobulins via their ability to bind immunoglobulin (Ig) constant regions (Chikaev et al. 2005 [PubMed 15676285]).[supplied by OMIM Mar 2008]

Gene Symbol:   FCRLB
Gene Name:   Fc receptor-like B
Aliases:   FCRL2, FCRLM2, FCRLY, FLJ31052, FREB-2, FcRY, RP11-474I16.6
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000162746
Entrez:   127943
Chromosome Mapping:   1q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 37.020000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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