PrimePCR™ Probe Assay: PITX2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PITX2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PITX2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053433
Assay Design:   exonic
Chromosome Location:   4:111539493-111539626question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000306732 ENST00000394598 ENST00000355080 ENST00000354925 ENST00000394595 ENST00000511837

Gene Information

This gene encodes a member of the RIEG/PITX homeobox family which is in the bicoid class of homeodomain proteins. The encoded protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. This protein plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes is involved in the development of the eye tooth and abdominal organs and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this gene are associated with Axenfeld-Rieger syndrome iridogoniodysgenesis syndrome and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   PITX2
Gene Name:   paired-like homeodomain 2
Aliases:   ARP1, Brx1, IDG2, IGDS, IGDS2, IHG2, IRID2, MGC111022, MGC20144, Otlx2, PTX2, RGS, RIEG, RIEG1, RS
RefSeq:   NC_000004.11 NT_016354.19 NG_007120.1
Ensembl:   ENSG00000164093
Entrez:   5308
Chromosome Mapping:   4q25

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