PrimePCR™ Probe Assay: GJB7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GJB7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GJB7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053481
Assay Design:   exonic
Chromosome Location:   6:87994452-87994567question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000525899 ENST00000369576 ENST00000296882

Gene Information

Connexins such as GJB7 are involved in the formation of gap junctions intercellular conduits that directly connect the cytoplasms of contacting cells. Each gap junction channel is formed by docking of 2 hemichannels each of which contains 6 connexin subunits (Sohl et al. 2003 [PubMed 12881038]).[supplied by OMIM Mar 2008]

Gene Symbol:   GJB7
Gene Name:   gap junction protein, beta 7, 25kDa
Aliases:   CX25, MGC71746, bA136M9.1, connexin25
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000164411
Entrez:   375519
Chromosome Mapping:   6q15

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