PrimePCR™ Probe Assay: SLC35A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $278.00
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PrimePCR™ PreAmp for Probe Assay: SLC35A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC35A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053482
Assay Design:   exonic
Chromosome Location:   6:88221296-88221432question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000369552 ENST00000369556 ENST00000544441

Gene Information

The protein encoded by this gene is found in the membrane of the Golgi apparatus where it transports nucleotide sugars into the Golgi. One such nucleotide sugar is CMP-sialic acid which is imported into the Golgi by the encoded protein and subsequently glycosylated. Defects in this gene are a cause of congenital disorder of glycosylation type 2F (CDG2F). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Dec 2009]

Gene Symbol:   SLC35A1
Gene Name:   solute carrier family 35 (CMP-sialic acid transporter), member A1
Aliases:   CDG2F, CMPST, CST, FLJ76955, hCST
RefSeq:   NC_000006.11 NT_007299.13 NG_016207.1
Ensembl:   ENSG00000164414
Entrez:   10559
Chromosome Mapping:   6q15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997000
y-intercept 35.810000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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