PrimePCR™ Probe Assay: ARHGAP12, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ARHGAP12, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ARHGAP12, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053557
Assay Design:   exonic
Chromosome Location:   10:32128634-32141457question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000311380 ENST00000375250 ENST00000344936 ENST00000454919 ENST00000396144 ENST00000375245

Gene Information

RHO GTPases (see ARHA MIM 165390) that participate in a wide variety of cellular processes are active in their GTP-bound form and inactive in their GDP-bound form. The hydrolysis of GTP and the inactivation of these GTPases are regulated by GTPase-activating proteins (GAPs) such as ARHGAP12 (Zhang et al. 2002 [PubMed 11854031]).[supplied by OMIM Mar 2008]

Gene Symbol:   ARHGAP12
Gene Name:   Rho GTPase activating protein 12
Aliases:   DKFZp779N2050, FLJ10971, FLJ20737, FLJ21785, FLJ45709
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000165322
Entrez:   94134
Chromosome Mapping:   10p11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.920000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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