PrimePCR™ Probe Assay: CLEC1B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CLEC1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CLEC1B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053583
Assay Design:   exonic
Chromosome Location:   12:10149473-10149585question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000398937 ENST00000428126 ENST00000298527 ENST00000348658

Gene Information

Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors such as CD94 (KLRD1; MIM 602894) and NKG2D (KLRC4; MIM 602893) that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 is a C-type lectin-like receptor expressed in myeloid cells and NK cells (Colonna et al. 2000 [PubMed 10671229]).[supplied by OMIM Jan 2011]

Gene Symbol:   CLEC1B
Gene Name:   C-type lectin domain family 1, member B
Aliases:   1810061I13Rik, CLEC2, CLEC2B, PRO1384, QDED721
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000165682
Entrez:   51266
Chromosome Mapping:   12p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.630000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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