PrimePCR™ Probe Assay: CYP2C19, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CYP2C19, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CYP2C19, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053595
Assay Design:   exonic
Chromosome Location:   10:96612575-96612676question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000371321 ENST00000260682

Gene Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize many xenobiotics including the anticonvulsive drug mephenytoin omeprazole diazepam and some barbiturates. Polymorphism within this gene is associated with variable ability to metabolize mephenytoin known as the poor metabolizer and extensive metabolizer phenotypes. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq Jul 2008]

Gene Symbol:   CYP2C19
Gene Name:   cytochrome P450, family 2, subfamily C, polypeptide 19
Aliases:   CPCJ, CYP2C, P450C2C, P450IIC19
RefSeq:   NC_000010.10 NG_008384.1 NT_030059.13
Ensembl:   ENSG00000165841
Entrez:   1557
Chromosome Mapping:   10q24

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