PrimePCR™ Probe Assay: FBN1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: FBN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FBN1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053616
Assay Design:   exonic
Chromosome Location:   15:48701317-48701430question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000316623

Gene Information

This gene encodes a member of the fibrillin family. The encoded protein is a large extracellular matrix glycoprotein that serve as a structural component of 10-12 nm calcium-binding microfibrils. These microfibrils provide force bearing structural support in elastic and nonelastic connective tissue throughout the body. Mutations in this gene are associated with Marfan syndrome isolated ectopia lentis autosomal dominant Weill-Marchesani syndrome MASS syndrome and Shprintzen-Goldberg craniosynostosis syndrome. [provided by RefSeq Jul 2008]

Gene Symbol:   FBN1
Gene Name:   fibrillin 1
Aliases:   FBN, MASS, MFS1, OCTD, SGS, SSKS, WMS
RefSeq:   NC_000015.9 NG_008805.2 NT_010194.17
Ensembl:   ENSG00000166147
Entrez:   2200
Chromosome Mapping:   15q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.290000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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