PrimePCR™ Probe Assay: WDR72, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: WDR72, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WDR72, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053640
Assay Design:   exonic
Chromosome Location:   15:53908224-53908349question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000396328 ENST00000557913 ENST00000360509 ENST00000559418 ENST00000560036

Gene Information

This gene encodes a protein with seven WD40 domains. Mutations in this gene have been associated with autosomal recessive hypomaturation amelogenesis imperfect type IIA3. [provided by RefSeq Aug 2011]

Gene Symbol:   WDR72
Gene Name:   WD repeat domain 72
Aliases:   AI2A3, FLJ38736, MGC126663, MGC126665
RefSeq:   NC_000015.9 NG_017034.1 NT_010194.17
Ensembl:   ENSG00000166415
Entrez:   256764
Chromosome Mapping:   15q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.280000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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