PrimePCR™ Probe Assay: PRKCB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PRKCB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRKCB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053649
Assay Design:   exonic
Chromosome Location:   16:24104191-24105504question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000321728 ENST00000303531 ENST00000498739

Gene Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions such as B cell activation apoptosis induction endothelial cell proliferation and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   PRKCB
Gene Name:   protein kinase C, beta
Aliases:   MGC41878, PKC-beta, PKCB, PRKCB1, PRKCB2
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000166501
Entrez:   5579
Chromosome Mapping:   16p11.2

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