PrimePCR™ Probe Assay: NAB2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NAB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NAB2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053676
Assay Design:   exonic
Chromosome Location:   12:57484975-57485086question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000300131 ENST00000342556 ENST00000357680

Gene Information

This gene encodes a member of the family of NGFI-A binding (NAB) proteins which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by the encoded protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex. Alternatively spliced transcript variants have been described but their biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   NAB2
Gene Name:   NGFI-A binding protein 2 (EGR1 binding protein 2)
Aliases:   MADER, MGC75085
RefSeq:   NC_000012.11 NG_021272.1 NT_029419.12
Ensembl:   ENSG00000166886
Entrez:   4665
Chromosome Mapping:   12q13.3

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