PrimePCR™ Probe Assay: ZNF668, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $270.00
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PrimePCR™ PreAmp for Probe Assay: ZNF668, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF668, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053717
Assay Design:   exonic
Chromosome Location:   16:31073422-31073588question
Amplicon Length:   137
Splice Variants Targeted:   ENST00000303660 ENST00000409487 ENST00000539609 ENST00000558170 ENST00000381569 ENST00000265801 ENST00000426488 ENST00000394983 ENST00000300849 ENST00000539836 ENST00000414399 ENST00000535577 ENST00000538906 ENST00000340622 ENST00000458143 ENST00000509479 ENST00000373324 ENST00000323301 ENST00000373322 ENST00000300417 ENST00000590071 ENST00000270288 ENST00000566786 ENST00000438926 ENST00000563236 ENST00000262502 ENST00000374278 ENST00000374276 ENST00000246229

Gene Information

Description Not Available

Gene Symbol:   ZNF668
Gene Name:   zinc finger protein 668
Aliases:   FLJ13479, FLJ14492
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000167394
Entrez:   79759
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.900000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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