PrimePCR™ Probe Assay: PSCA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PSCA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PSCA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053738
Assay Design:   exonic
Chromosome Location:   8:143763591-143763681question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000571412 ENST00000361134 ENST00000538474 ENST00000374796 ENST00000359003 ENST00000216218 ENST00000551848 ENST00000276241 ENST00000344129 ENST00000361352 ENST00000449321 ENST00000392744 ENST00000374789 ENST00000374788 ENST00000346874 ENST00000374794 ENST00000350537 ENST00000374790 ENST00000545693 ENST00000545260 ENST00000374161 ENST00000325239 ENST00000413659 ENST00000442544 ENST00000581580 ENST00000396925 ENST00000539278 ENST00000403662 ENST00000262825 ENST00000346436 ENST00000376497 ENST

Gene Information

This gene encodes a glycosylphosphatidylinositol-anchored cell membrane glycoprotein. In addition to being highly expressed in the prostate it is also expressed in the bladder placenta colon kidney and stomach. This gene is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. This gene includes a polymorphism that results in an upstream start codon in some individuals; this polymorphism is thought to be associated with a risk for certain gastric and bladder cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq Feb 2010]

Gene Symbol:   PSCA
Gene Name:   prostate stem cell antigen
Aliases:   PRO232
RefSeq:   NC_000008.10 NG_011722.2 NT_008046.16
Ensembl:   ENSG00000167653
Entrez:   8000
Chromosome Mapping:   8q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.190000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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