PrimePCR™ Probe Assay: NDUFV1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NDUFV1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NDUFV1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053754
Assay Design:   exonic
Chromosome Location:   11:67376191-67377024question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000322776 ENST00000532303 ENST00000532244 ENST00000529927 ENST00000532343 ENST00000415352 ENST00000533075 ENST00000529867 ENST00000530638 ENST00000528314

Gene Information

The mitochondrial respiratory chain provides energy to cells via oxidative phosphorylation and consists of four membrane-bound electron-transporting protein complexes (I-IV) and an ATP synthase (complex V). This gene encodes a 51 kDa subunit of the NADH:ubiquinone oxidoreductase complex I; a large complex with at least 45 nuclear and mitochondrial encoded subunits that liberates electrons from NADH and channels them to ubiquinone. This subunit carries the NADH-binding site as well as flavin mononucleotide (FMN)- and Fe-S-biding sites. Defects in complex I are a common cause of mitochondrial dysfunction; a syndrome that occurs in approximately 1 in 10000 live births. Mitochondrial complex I deficiency is linked to myopathies encephalomyopathies and neurodegenerative disorders such as Parkinson's disease and Leigh syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq Oct 2009]

Gene Symbol:   NDUFV1
Gene Name:   NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa
Aliases:   CI-51K, CI51KD, FLJ59059, UQOR1
RefSeq:   NC_000011.9 NT_167190.1 NG_013353.1
Ensembl:   ENSG00000167792
Entrez:   4723
Chromosome Mapping:   11q13

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