PrimePCR™ Probe Assay: RAB31, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RAB31, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RAB31, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053815
Assay Design:   exonic
Chromosome Location:   18:9861683-9861832question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000578921 ENST00000306096

Gene Information

Small GTP-binding proteins of the RAB family such as RAB31 play essential roles in vesicle and granule targeting (Bao et al. 2002 [PubMed 11784320]).[supplied by OMIM Jul 2009]

Gene Symbol:   RAB31
Gene Name:   RAB31, member RAS oncogene family
Aliases:   Rab22B
RefSeq:   NC_000018.9 NT_010859.14
Ensembl:   ENSG00000168461
Entrez:   11031
Chromosome Mapping:   18p11.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.700000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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