PrimePCR™ Probe Assay: B3GALNT1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: B3GALNT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: B3GALNT1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053882
Assay Design:   exonic
Chromosome Location:   3:160804456-160804563question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000374747 ENST00000420886 ENST00000267176 ENST00000320474 ENST00000392779 ENST00000392781 ENST00000473285 ENST00000417187 ENST00000488170 ENST00000468268 ENST00000492353 ENST00000473142 ENST00000498216 ENST00000484127 ENST00000460353 ENST00000494173 ENST00000392780 ENST00000532828 ENST00000527838 ENST00000455627 ENST00000397850 ENST00000302347 ENST00000476854 ENST00000494103 ENST00000404366 ENST00000469863 ENST00000458031 ENST00000380773 ENST00000380771 ENST00000380769 ENST00000380764 ENST

Gene Information

This gene is a member of the beta-13-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine galactose N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1 whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3 beta3GalT5). The encoded protein of this gene does not use N-acetylglucosamine as an acceptor sugar at all. Multiple transcript variants that are alternatively spliced in the 5' UTR have been described; they all encode the same protein. [provided by RefSeq Jul 2008]

Gene Symbol:   B3GALNT1
Gene Name:   beta-1,3-N-acetylgalactosaminyltransferase 1 (globoside blood group)
Aliases:   B3GALT3, GLCT3, GLOB, Gb4Cer, P, P1, beta3Gal-T3, galT3
RefSeq:   NC_000003.11 NT_005612.16 NG_007854.1
Ensembl:   ENSG00000169255
Entrez:   8706
Chromosome Mapping:   3q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.660000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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