PrimePCR™ Probe Assay: NPR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NPR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NPR1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053892
Assay Design:   exonic
Chromosome Location:   1:153665989-153666111question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000368680 ENST00000368677

Gene Information

Guanylyl cyclases catalyzing the production of cGMP from GTP are classified as soluble and membrane forms (Garbers and Lowe 1994 [PubMed 7982997]). The membrane guanylyl cyclases often termed guanylyl cyclases A through F form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain a single membrane-spanning domain and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961). Also see NPR3 (MIM 108962) which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295) respectively).[supplied by OMIM May 2009]

Gene Symbol:   NPR1
Gene Name:   natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A)
Aliases:   ANPRA, ANPa, GUC2A, GUCY2A, NPRA
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000169418
Entrez:   4881
Chromosome Mapping:   1q21-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.860000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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