PrimePCR™ Probe Assay: KCNK9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KCNK9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: KCNK9, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053893
Assay Design:   exonic
Chromosome Location:   8:140630524-140630636question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000303015 ENST00000520439

Gene Information

This gene encodes a member of the superfamily of potassium channel proteins containing two pore-forming P domains and is highly expressed in the cerebellum. Amplification and overexpression of this gene has been observed in several types of human carcinomas notably in breast cancer. This gene is imprinted in fetal brain with preferential expression from the maternal allele. [provided by RefSeq Oct 2010]

Gene Symbol:   KCNK9
Gene Name:   potassium channel, subfamily K, member 9
Aliases:   K2p9.1, KT3.2, MGC138268, MGC138270, TASK-3, TASK3
RefSeq:   NC_000008.10 NG_012842.1 NT_008046.16
Ensembl:   ENSG00000169427
Entrez:   51305
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.050000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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