PrimePCR™ Probe Assay: GSTA4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GSTA4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GSTA4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054011
Assay Design:   exonic
Chromosome Location:   6:52847486-52849370question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000370963 ENST00000370960 ENST00000370959 ENST00000457564 ENST00000541324

Gene Information

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic carcinogenic and pharmacologically active electrophilic compounds. At present eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha kappa mu omega pi sigma theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes which are located in a cluster on chromosome 6 are highly related and encode enzymes with glutathione peroxidase activity that function in the detoxification of lipid peroxidation products. Reactive electrophiles produced by oxidative metabolism have been linked to a number of degenerative diseases including Parkinson's disease Alzheimer's disease cataract formation and atherosclerosis. [provided by RefSeq Jul 2008]

Gene Symbol:   GSTA4
Gene Name:   glutathione S-transferase alpha 4
Aliases:   DKFZp686D21185, GSTA4-4, GTA4
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000170899
Entrez:   2941
Chromosome Mapping:   6p12.1

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