PrimePCR™ Probe Assay: HOPX, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HOPX, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HOPX, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054067
Assay Design:   exonic
Chromosome Location:   4:57516954-57522110question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000508121 ENST00000556376 ENST00000553379 ENST00000381260 ENST00000381255 ENST00000317745 ENST00000503639 ENST00000503864 ENST00000337881 ENST00000555760 ENST00000556614 ENST00000509435 ENST00000514890 ENST00000506661 ENST00000557328 ENST00000420433 ENST00000554144

Gene Information

The protein encoded by this gene is a homeodomain protein that lacks certain conserved residues required for DNA binding. It was reported that choriocarcinoma cell lines and tissues failed to express this gene which suggested the possible involvement of this gene in malignant conversion of placental trophoblasts. Studies in mice suggest that this protein may interact with serum response factor (SRF) and modulate SRF-dependent cardiac-specific gene expression and cardiac development. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq Feb 2009]

Gene Symbol:   HOPX
Gene Name:   HOP homeobox
Aliases:   CAMEO, HOD, HOP, LAGY, MGC20820, NECC1, OB1, SMAP31, TOTO
RefSeq:   NC_000004.11 NT_022853.15
Ensembl:   ENSG00000171476
Entrez:   84525
Chromosome Mapping:   4q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.820000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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