PrimePCR™ Probe Assay: BPTF, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BPTF, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BPTF, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054089
Assay Design:   exonic
Chromosome Location:   17:65941790-65941912question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000278407 ENST00000340687 ENST00000378323 ENST00000378324 ENST00000403558 ENST00000544778 ENST00000306378 ENST00000424123 ENST00000580465 ENST00000342579 ENST00000321892 ENST00000335221 ENST00000573838 ENST00000573834 ENST00000574648 ENST00000579610 ENST00000575874

Gene Information

This gene was identified by the reactivity of its encoded protein to a monoclonal antibody prepared against brain homogenates from patients with Alzheimer's disease. Analysis of the original protein (fetal Alz-50 reactive clone 1 or FAC1) identified as an 810 aa protein containing a DNA-binding domain and a zinc finger motif suggested it might play a role in the regulation of transcription. High levels of FAC1 were detected in fetal brain and in patients with neurodegenerative diseases. The protein encoded by this gene is actually much larger than originally thought and it also contains a C-terminal bromodomain characteristic of proteins that regulate transcription during proliferation. The encoded protein is highly similar to the largest subunit of the Drosophila NURF (nucleosome remodeling factor) complex. In Drosophila the NURF complex which catalyzes nucleosome sliding on DNA and interacts with sequence-specific transcription factors is necessary for the chromatin remodeling required for transcription. Two alternative transcripts encoding different isoforms have been described completely. [provided by RefSeq Jul 2008]

Gene Symbol:   BPTF
Gene Name:   bromodomain PHD finger transcription factor
Aliases:   FAC1, FALZ, NURF301
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000171634
Entrez:   2186
Chromosome Mapping:   17q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997000
y-intercept 36.030000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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