PrimePCR™ Probe Assay: COQ2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: COQ2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: COQ2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054215
Assay Design:   exonic
Chromosome Location:   4:84188772-84188915question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000311469 ENST00000311461 ENST00000439031

Gene Information

This gene encodes an enzyme that functions in the final steps in the biosynthesis of CoQ (ubiquinone) a redox carrier in the mitochondrial respiratory chain and a lipid-soluble antioxidant. This enzyme which is part of the coenzyme Q10 pathway catalyzes the prenylation of parahydroxybenzoate with an all-trans polyprenyl group. Mutations in this gene cause coenzyme Q10 deficiency a mitochondrial encephalomyopathy and also COQ2 nephropathy an inherited form of mitochondriopathy with primary renal involvement. [provided by RefSeq Oct 2009]

Gene Symbol:   COQ2
Gene Name:   coenzyme Q2 homolog, prenyltransferase (yeast)
Aliases:   CL640, FLJ13014, FLJ26072
RefSeq:   NC_000004.11 NG_015825.1 NT_016354.19
Ensembl:   ENSG00000173085
Entrez:   27235
Chromosome Mapping:   4q21.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.370000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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