PrimePCR™ Probe Assay: GNG5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GNG5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GNG5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054288
Assay Design:   exonic
Chromosome Location:   1:84972099-84972198question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000370645 ENST00000370641

Gene Information

G proteins are trimeric (alpha-beta-gamma) membrane-associated proteins that regulate flow of information from cell surface receptors to a variety of internal metabolic effectors. Interaction of a G protein with its activated receptor promotes exchange of GTP for GDP that is bound to the alpha subunit. The alpha-GTP complex dissociates from the beta-gamma heterodimer so that the subunits in turn may interact with and regulate effector molecules (Gilman 1987 [PubMed 3113327]; summary by Ahmad et al. 1995) [PubMed 7606925].[supplied by OMIM Nov 2010]

Gene Symbol:   GNG5
Gene Name:   guanine nucleotide binding protein (G protein), gamma 5
Aliases:   FLJ92393
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000174021
Entrez:   2787
Chromosome Mapping:   1p22

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