PrimePCR™ Probe Assay: APITD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: APITD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: APITD1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054359
Assay Design:   exonic
Chromosome Location:   1:10490601-10493968question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000309048 ENST00000477755 ENST00000602787 ENST00000602296 ENST00000400900 ENST00000470413

Gene Information

This gene was identified in the neuroblastoma tumor suppressor candidate region on chromosome 1p36. It contains a TFIID-31 domain similar to that found in TATA box-binding protein-associated factor TAF(II)31 which is required for p53-mediated transcription activation. This gene was expressed at very low levels in neuroblastoma tumors and was shown to reduce cell growth in neuroblastoma cells suggesting that it may have a role in a cell death pathway. The protein is a component of multiple complexes including the Fanconi anemia (FA) core complex the APITD1/CENPS complex and the CENPA-CAD (nucleosome distal) complex. Known functions include an involvement with chromatin associations of the FA core complex and a role in the stable assembly of the outer kinetochore. Alternative splicing of this gene results in multiple transcript variants. Naturally occurring read-through transcripts also exist between this gene and the downstream cortistatin (CORT) gene as represented in GeneID:100526739. An APITD1-related pseudogene has been identified on chromosome 7. [provided by RefSeq Nov 2010]

Gene Symbol:   APITD1
Gene Name:   apoptosis-inducing, TAF9-like domain 1
Aliases:   CENP-S, CENPS, MGC32686, MHF1
RefSeq:   Not Available
Ensembl:   ENSG00000175279
Entrez:   378708
Chromosome Mapping:   1p36.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.600000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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