PrimePCR™ Probe Assay: LPL, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LPL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LPL, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054367
Assay Design:   exonic
Chromosome Location:   8:19805729-19805832question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000520959 ENST00000524029 ENST00000522701 ENST00000311322

Gene Information

LPL encodes lipoprotein lipase which is expressed in heart muscle and adipose tissue. LPL functions as a homodimer and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq Jul 2008]

Gene Symbol:   LPL
Gene Name:   lipoprotein lipase
Aliases:   HDLCQ11, LIPD
RefSeq:   NC_000008.10 NG_008855.1 NT_167187.1
Ensembl:   ENSG00000175445
Entrez:   4023
Chromosome Mapping:   8p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.240000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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