PrimePCR™ Probe Assay: PVRL3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PVRL3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PVRL3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054525
Assay Design:   exonic
Chromosome Location:   3:110791022-110831014question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000491525 ENST00000461477 ENST00000485303 ENST00000319792 ENST00000486596 ENST00000493615 ENST00000481766

Gene Information

This gene encodes a member of the nectin family of proteins which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin a filamentous actin-binding protein involved in the regulation of directional motility cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq Aug 2011]

Gene Symbol:   PVRL3
Gene Name:   poliovirus receptor-related 3
Aliases:   CD113, CDw113, DKFZp566B0846, FLJ90624, PPR3, PRR3, PVRR3, nectin-3
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000177707
Entrez:   25945
Chromosome Mapping:   3q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997200
y-intercept 36.000000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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