PrimePCR™ Probe Assay: FLII, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FLII, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FLII, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054526
Assay Design:   exonic
Chromosome Location:   17:18158102-18158519question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000251334 ENST00000547562 ENST00000374408 ENST00000301061 ENST00000578558 ENST00000327031 ENST00000545457 ENST00000579294 ENST00000488932 ENST00000581349 ENST00000473425 ENST00000379450 ENST00000395894 ENST00000464847 ENST00000421016 ENST00000521903 ENST00000449803 ENST00000329047 ENST00000427180 ENST00000541152 ENST00000427674 ENST00000423034 ENST00000431235 ENST00000427177 ENST00000574891 ENST00000576289 ENST00000571241 ENST00000576977 ENST00000573468 ENST00000574362 ENST00000575088 ENST

Gene Information

This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq Jul 2008]

Gene Symbol:   FLII
Gene Name:   flightless I homolog (Drosophila)
Aliases:   FLI, FLIL, Fli1, MGC39265
RefSeq:   NC_000017.10 NG_023243.1 NT_010718.16
Ensembl:   ENSG00000177731
Entrez:   2314
Chromosome Mapping:   17p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.540000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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