PrimePCR™ Probe Assay: GPBAR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GPBAR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GPBAR1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054653
Assay Design:   exonic
Chromosome Location:   2:219128409-219128501question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000479077 ENST00000522678 ENST00000519574 ENST00000521462

Gene Information

This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP activation of a MAP kinase signaling pathway and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq Jul 2008]

Gene Symbol:   GPBAR1
Gene Name:   G protein-coupled bile acid receptor 1
Aliases:   BG37, GPCR19, GPR131, M-BAR, MGC40597, TGR5
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000179921
Entrez:   151306
Chromosome Mapping:   2q35

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