PrimePCR™ Probe Assay: TH, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054667
Assay Design:   exonic
Chromosome Location:   11:2186930-2187257question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000333684 ENST00000412076 ENST00000352909 ENST00000381175 ENST00000381178 ENST00000295269 ENST00000424985 ENST00000299164 ENST00000300105 ENST00000360319 ENST00000369850 ENST00000344736 ENST00000422373 ENST00000276594 ENST00000526589 ENST00000366584 ENST00000366582 ENST00000596447 ENST00000597475 ENST00000600520 ENST00000599487

Gene Information

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   TH
Gene Name:   tyrosine hydroxylase
Aliases:   DYT14, DYT5b, TYH
RefSeq:   NC_000011.9 NG_007114.1 NT_009237.18 NG_008128.1
Ensembl:   ENSG00000180176
Entrez:   7054
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.320000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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