PrimePCR™ Probe Assay: RP4-604K5.1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RP4-604K5.1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RP4-604K5.1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054847
Assay Design:   exonic
Chromosome Location:   1:87329191-87333755question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000331835 ENST00000370554 ENST00000401030

Gene Information

This gene encodes a selenoprotein which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure the sec insertion sequence (SECIS) that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Studies in mouse suggest that this selenoprotein may have redox function and may be involved in the quality control of protein folding. This gene is localized on chromosome 1p31 a genetic locus commonly mutated or deleted in human cancers. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   RP4-604K5.1
Gene Name:   15 kDa selenoprotein
Aliases:   Not Available
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000183291
Entrez:   9403
Chromosome Mapping:   1p31

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