PrimePCR™ Probe Assay: ASB7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ASB7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: ASB7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054857
Assay Design:   exonic
Chromosome Location:   15:101152402-101152542question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000332783 ENST00000558747 ENST00000343276

Gene Information

The protein encoded by this gene belongs to a family of ankyrin repeat proteins that along with four other protein families contains a C-terminal SOCS box motif. Growing evidence suggests that the SOCS box acts as a bridge between specific substrate-binding domains and the more generic proteins that comprise a large family of E3 ubiquitin protein ligases. In this way SOCS box containing proteins may regulate protein turnover by targeting proteins for polyubiquination and therefore for proteasome-mediated degradation. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   ASB7
Gene Name:   ankyrin repeat and SOCS box containing 7
Aliases:   FLJ22551, FLJ25192
RefSeq:   NC_000015.9 NT_010274.17
Ensembl:   ENSG00000183475
Entrez:   140460
Chromosome Mapping:   -

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.700000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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