PrimePCR™ Probe Assay: CLDN5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CLDN5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CLDN5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054904
Assay Design:   exonic
Chromosome Location:   22:19511467-19511682question
Amplicon Length:   186
Splice Variants Targeted:   ENST00000548421 ENST00000406028 ENST00000403084 ENST00000413119

Gene Information

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Aug 2008]

Gene Symbol:   CLDN5
Gene Name:   claudin 5
Aliases:   AWAL, BEC1, CPETRL1, TMVCF
RefSeq:   NC_000022.10 NT_011519.10
Ensembl:   ENSG00000184113
Entrez:   7122
Chromosome Mapping:   22q11.21

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