PrimePCR™ Probe Assay: COL14A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: COL14A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: COL14A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055198
Assay Design:   exonic
Chromosome Location:   8:121222009-121222118question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000309791 ENST00000297848 ENST00000523142 ENST00000434620 ENST00000247781 ENST00000537875

Gene Information

Type XIV collagen is a fibril-associated collagen with an interrupted triple helix. It interacts with the fibril surface and regulates fibrillogenesis (Ansorge et al. 2009 [PubMed 19136672]).[supplied by OMIM Aug 2010]

Gene Symbol:   COL14A1
Gene Name:   collagen, type XIV, alpha 1
Aliases:   UND
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000187955
Entrez:   7373
Chromosome Mapping:   8q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.470000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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