PrimePCR™ Probe Assay: NOC2L, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $278.00
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PrimePCR™ PreAmp for Probe Assay: NOC2L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NOC2L, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055270
Assay Design:   exonic
Chromosome Location:   1:894384-894606question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000314134 ENST00000371675 ENST00000432842 ENST00000371663 ENST00000357466 ENST00000311502 ENST00000412055 ENST00000448120 ENST00000528431 ENST00000394955 ENST00000430933 ENST00000429699 ENST00000421229 ENST00000454774 ENST00000419412 ENST00000552068 ENST00000550384 ENST00000324771 ENST00000418800 ENST00000454612 ENST00000376569 ENST00000376568 ENST00000508312 ENST00000376567 ENST00000513240 ENST00000376575 ENST00000376570 ENST00000452441 ENST00000446312 ENST00000412329 ENST00000449518 ENST

Gene Information

Histone modification by histone acetyltransferases (HAT) and histone deacetylases (HDAC) can control major aspects of transcriptional regulation. NOC2L represents a novel HDAC-independent inhibitor of histone acetyltransferase (INHAT) (Hublitz et al. 2005 [PubMed 16322561]).[supplied by OMIM Mar 2008]

Gene Symbol:   NOC2L
Gene Name:   nucleolar complex associated 2 homolog (S. cerevisiae)
Aliases:   DKFZp564C186, FLJ35172, NET15, NET7, NIR
RefSeq:   NC_000001.10 NG_027546.1 NT_004350.19
Ensembl:   ENSG00000188976
Entrez:   26155
Chromosome Mapping:   1p36.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.150000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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