PrimePCR™ Probe Assay: SCN8A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SCN8A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SCN8A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055390
Assay Design:   exonic
Chromosome Location:   12:52099202-52099345question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000550891 ENST00000354534 ENST00000355133 ENST00000551216 ENST00000545061

Gene Information

This gene encodes a member of the sodium channel alpha subunit gene family. The encoded protein forms the ion pore region of the voltage-gated sodium channel. This protein is essential for the rapid membrane depolarization that occurs during the formation of the action potential in excitable neurons. Mutations in this gene are associated with mental retardation pancerebellar atrophy and ataxia. Alternate splicing results in multiple transcript variants.[provided by RefSeq May 2010]

Gene Symbol:   SCN8A
Gene Name:   sodium channel, voltage gated, type VIII, alpha subunit
Aliases:   CERIII, MED, NaCh6, Nav1.6, PN4
RefSeq:   NC_000012.11 NT_029419.12 NG_021180.1
Ensembl:   ENSG00000196876
Entrez:   6334
Chromosome Mapping:   12q13

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