PrimePCR™ Probe Assay: SLC39A10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SLC39A10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC39A10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055395
Assay Design:   exonic
Chromosome Location:   2:196545006-196545147question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000458054 ENST00000409086 ENST00000359634 ENST00000418005

Gene Information

Zinc is an essential cofactor for hundreds of enzymes. It is involved in protein nucleic acid carbohydrate and lipid metabolism as well as in the control of gene transcription growth development and differentiation. SLC39A10 belongs to a subfamily of proteins that show structural characteristics of zinc transporters (Taylor and Nicholson 2003 [PubMed 12659941]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC39A10
Gene Name:   solute carrier family 39 (zinc transporter), member 10
Aliases:   DKFZp781L10106, LZT-Hs2, MGC126565, MGC138428
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000196950
Entrez:   57181
Chromosome Mapping:   2q32.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.210000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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