PrimePCR™ Probe Assay: LAGE3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LAGE3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LAGE3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055400
Assay Design:   exonic
Chromosome Location:   X:153706121-153706249question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000407062 ENST00000357360 ENST00000601651

Gene Information

This gene belongs to the ESO/LAGE gene family members of which are clustered together on chromosome Xq28 and have similar exon-intron structures. Unlike the other family members which are normally expressed only in testis and activated in a wide range of human tumors this gene is ubiquitously expressed in somatic tissues. The latter combined with the finding that it is highly conserved in mouse and rat suggests that the encoded protein is functionally important. An intronless pseudogene with high sequence similarity to this gene is located on chromosome 9. [provided by RefSeq Jul 2008]

Gene Symbol:   LAGE3
Gene Name:   L antigen family, member 3
Aliases:   CVG5, DXS9879E, DXS9951E, ESO3, ITBA2
RefSeq:   NC_000023.10 NT_167198.1
Ensembl:   ENSG00000196976
Entrez:   8270
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.670000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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