PrimePCR™ Probe Assay: ZNF675, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ZNF675, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF675, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055446
Assay Design:   exonic
Chromosome Location:   19:23869901-23869990question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000379145 ENST00000597424 ENST00000596053 ENST00000600692 ENST00000358296 ENST00000300619 ENST00000601935 ENST00000600313 ENST00000596211 ENST00000359788 ENST00000601010 ENST00000599168 ENST00000339642 ENST00000562085

Gene Information

Description Not Available

Gene Symbol:   ZNF675
Gene Name:   zinc finger protein 675
Aliases:   FLJ36350, TBZF, TIZ
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000197372
Entrez:   171392
Chromosome Mapping:   19p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.660000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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