PrimePCR™ Probe Assay: VEPH1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: VEPH1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: VEPH1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055454
Assay Design:   exonic
Chromosome Location:   3:157213051-157213198question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000392833 ENST00000362010 ENST00000392832 ENST00000494677 ENST00000468233 ENST00000487753 ENST00000461299 ENST00000489602 ENST00000543418 ENST00000537559

Gene Information

Description Not Available

Gene Symbol:   VEPH1
Gene Name:   ventricular zone expressed PH domain homolog 1 (zebrafish)
Aliases:   FLJ12604, KIAA1692, MELT, MGC111426, MGC126709, MGC142115, VEPH
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000197415
Entrez:   79674
Chromosome Mapping:   3q24-q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.590000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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