PrimePCR™ Probe Assay: COL13A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: COL13A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: COL13A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055461
Assay Design:   exonic
Chromosome Location:   10:71690206-71692325question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000359314 ENST00000356083 ENST00000409891 ENST00000255305 ENST00000400602 ENST00000262384 ENST00000398978 ENST00000354547 ENST00000357811 ENST00000520267 ENST00000517713 ENST00000522165 ENST00000520133 ENST00000398975 ENST00000456019 ENST00000398974 ENST00000398971 ENST00000398968 ENST00000398966 ENST00000398964 ENST00000398969 ENST00000356340 ENST00000398972 ENST00000398973

Gene Information

This gene encodes the alpha chain of one of the nonfibrillar collagens. The function of this gene product is not known however it has been detected at low levels in all connective tissue-producing cells so it may serve a general function in connective tissues. Unlike most of the collagens which are secreted into the extracellular matrix collagen XIII contains a transmembrane domain and the protein has been localized to the plasma membrane. The transcripts for this gene undergo complex and extensive splicing involving at least eight exons. Like other collagens collagen XIII is a trimer; it is not known whether this trimer is composed of one or more than one alpha chain isomer. A number of alternatively spliced transcript variants have been described but the full length nature of some of them has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   COL13A1
Gene Name:   collagen, type XIII, alpha 1
Aliases:   COLXIIIA1, FLJ42485
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000197467
Entrez:   1305
Chromosome Mapping:   10q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.992000
y-intercept 37.700000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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