PrimePCR™ Probe Assay: GZMM, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GZMM, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GZMM, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055466
Assay Design:   exonic
Chromosome Location:   19:549736-549840question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000456197 ENST00000446306 ENST00000355540 ENST00000359863 ENST00000566839 ENST00000570190 ENST00000264553 ENST00000262525 ENST00000254853 ENST00000512825 ENST00000424747 ENST00000328070 ENST00000265310 ENST00000439304 ENST00000308284 ENST00000223210 ENST00000374168 ENST00000374166 ENST00000526792 ENST00000530003 ENST00000531382 ENST00000374162 ENST00000319988 ENST00000565218 ENST00000562259 ENST00000562468 ENST00000561982

Gene Information

Human natural killer (NK) cells and activated lymphocytes express and store a distinct subset of neutral serine proteases together with proteoglycans and other immune effector molecules in large cytoplasmic granules. These serine proteases are collectively termed granzymes and include 4 distinct gene products: granzyme A granzyme B granzyme H and Met-ase also known as granzyme M. [provided by RefSeq Jul 2008]

Gene Symbol:   GZMM
Gene Name:   granzyme M (lymphocyte met-ase 1)
Aliases:   LMET1, MET1
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000197540
Entrez:   3004
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997100
y-intercept 36.890000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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