PrimePCR™ Probe Assay: S100A6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: S100A6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: S100A6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055516
Assay Design:   exonic
Chromosome Location:   1:153507239-153507701question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000246801 ENST00000307145 ENST00000288670 ENST00000381241 ENST00000381229 ENST00000381233 ENST00000454575 ENST00000340370 ENST00000251157 ENST00000283256 ENST00000375437 ENST00000357398 ENST00000341446 ENST00000296140 ENST00000410053 ENST00000404136 ENST00000295749 ENST00000029410 ENST00000275732 ENST00000311172 ENST00000409314 ENST00000409418 ENST00000355522 ENST00000262085 ENST00000496817 ENST00000368719 ENST00000368720 ENST00000462776 ENST00000356387 ENST00000457953 ENST00000372421 ENST

Gene Information

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release stimulation of prolactin secretion and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma. [provided by RefSeq Jul 2008]

Gene Symbol:   S100A6
Gene Name:   S100 calcium binding protein A6
Aliases:   2A9, 5B10, CABP, CACY, PRA
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000197956
Entrez:   6277
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.400000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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