PrimePCR™ Probe Assay: CSF2RA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CSF2RA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CSF2RA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055553
Assay Design:   exonic
Chromosome Location:   X:1407525-1407735question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000381529 ENST00000381524 ENST00000412290 ENST00000419094 ENST00000381509 ENST00000355805 ENST00000355432 ENST00000381500 ENST00000432318 ENST00000417535 ENST00000361536 ENST00000501036

Gene Information

The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2 a cytokine which controls the production differentiation and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene with some of the isoforms being membrane-bound and others being soluble. [provided by RefSeq Jul 2008]

Gene Symbol:   CSF2RA
Gene Name:   colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
Aliases:   CD116, CDw116, CSF2R, CSF2RAX, CSF2RAY, CSF2RX, CSF2RY, GM-CSF-R-alpha, GMCSFR, GMR, MGC3848, MGC4838
RefSeq:   NC_000023.10 NC_000024.9 NT_167196.1 NT_167204.1 NG_012280.1
Ensembl:   ENSG00000198223
Entrez:   1438
Chromosome Mapping:   Xp22.32 and Yp11.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.840000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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