PrimePCR™ Probe Assay: LGR4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LGR4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LGR4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055755
Assay Design:   exonic
Chromosome Location:   11:27406826-27406949question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000379214 ENST00000389858 ENST00000480977

Gene Information

G protein-coupled receptors (GPCRs) play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family such as GPR48 have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain (Weng et al. 2008 [PubMed 18424556]).[supplied by OMIM Aug 2008]

Gene Symbol:   LGR4
Gene Name:   leucine-rich repeat containing G protein-coupled receptor 4
Aliases:   GPR48
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000205213
Entrez:   55366
Chromosome Mapping:   11p14-p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.150000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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