PrimePCR™ Probe Assay: ORM1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $270.00
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PrimePCR™ PreAmp for Probe Assay: ORM1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ORM1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055966
Assay Design:   exonic
Chromosome Location:   9:117087073-117087165question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000259396 ENST00000431067 ENST00000412657 ENST00000334564 ENST00000371600 ENST00000522208

Gene Information

This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however it may be involved in aspects of immunosuppression. [provided by RefSeq Jul 2008]

Gene Symbol:   ORM1
Gene Name:   orosomucoid 1
Aliases:   AGP-A, AGP1, ORM
RefSeq:   NC_000009.11 NG_012108.1 NT_008470.19
Ensembl:   ENSG00000229314
Entrez:   5004
Chromosome Mapping:   9q31-q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.160000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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