PrimePCR™ Probe Assay: PSG2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $278.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: PSG2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PSG2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0056040
Assay Design:   exonic
Chromosome Location:   19:43568629-43570684question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000406487 ENST00000407356 ENST00000407568 ENST00000599812 ENST00000342951

Gene Information

The human pregnancy-specific glycoproteins (PSGs) are a family of proteins that are synthesized in large amounts by placental trophoblasts and released into the maternal circulation during pregnancy. Molecular cloning and analysis of several PSG genes has indicated that the PSGs form a subgroup of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily of genes. Members of the CEA family consist of a single N domain with structural similarity to the immunoglobulin variable domains followed by a variable number of immunoglobulin constant-like A and/or B domains. Most PSGs have an arg-gly-asp (RGD) motif which has been shown to function as an adhesion recognition signal for several integrins in the N-terminal domain (summary by Teglund et al. 1994 [PubMed 7851896]). For additional general information about the PSG gene family see PSG1 (MIM 176390).[supplied by OMIM Oct 2009]

Gene Symbol:   PSG2
Gene Name:   pregnancy specific beta-1-glycoprotein 2
Aliases:   CEA, PSBG2, PSG1, PSGGB
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000242221
Entrez:   5670
Chromosome Mapping:   19q13.1-q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 35.720000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download