PrimePCR™ Probe Assay: LYN, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0056127
Assay Design:   exonic
Chromosome Location:   8:56922830-56922941question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000519728 ENST00000520220

Gene Information

This gene encodes a tyrosine protein kinase which maybe involved in the regulation of mast cell degranulation and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2011]

Gene Symbol:   LYN
Gene Name:   v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
Aliases:   FLJ26625, JTK8
RefSeq:   NC_000008.10 NT_008183.19
Ensembl:   ENSG00000254087
Entrez:   4067
Chromosome Mapping:   8q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.940000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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