PrimePCR™ Probe Assay: NEDD8-MDP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $278.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: NEDD8-MDP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NEDD8-MDP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0056160
Assay Design:   exonic
Chromosome Location:   14:24687623-24701558question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000451075 ENST00000435344 ENST00000477737 ENST00000243878 ENST00000220764 ENST00000298694 ENST00000298693 ENST00000361246 ENST00000373091 ENST00000370092 ENST00000217901 ENST00000370093 ENST00000427365 ENST00000238091 ENST00000355346 ENST00000534348 ENST00000250495 ENST00000524927 ENST00000377311 ENST00000439068 ENST00000301466 ENST00000382554 ENST00000600403 ENST00000593996 ENST00000598838 ENST00000596495

Gene Information

This locus represents naturally occurring read-through transcription between the neighboring NEDD8 (neural precursor cell expressed developmentally down-regulated 8) and MDP1 (magnesium-dependent phosphatase 1) genes on chromosome 14. The read-through transcript encodes a fusion protein that shares sequence identity with the products of each individual gene. [provided by RefSeq Dec 2010]

Gene Symbol:   NEDD8-MDP1
Gene Name:   NEDD8-MDP1 readthrough
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000255526
Entrez:   100528064
Chromosome Mapping:   14q

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.270000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download