PrimePCR™ Probe Assay: MGAM, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MGAM, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MGAM, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0056192
Assay Design:   exonic
Chromosome Location:   7:141708392-141708500question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000320230 ENST00000552656 ENST00000394013 ENST00000345093 ENST00000389749 ENST00000299202 ENST00000262178 ENST00000402066 ENST00000465654 ENST00000549489 ENST00000497673 ENST00000475668 ENST00000216271 ENST00000448072 ENST00000349505 ENST00000371561 ENST00000350902 ENST00000371550 ENST00000371546 ENST00000371555 ENST00000371553 ENST00000371559 ENST00000371560 ENST00000315048 ENST00000369448 ENST00000271751 ENST00000367007 ENST00000355235 ENST00000356834 ENST00000524222 ENST00000569657 ENST

Gene Information

This gene encodes maltase-glucoamylase which is a brush border membrane enzyme that plays a role in the final steps of digestion of starch. The protein has two catalytic sites identical to those of sucrase-isomaltase but the proteins are only 59% homologous. Both are members of glycosyl hydrolase family 31 which has a variety of substrate specificities. [provided by RefSeq Jul 2008]

Gene Symbol:   MGAM
Gene Name:   maltase-glucoamylase (alpha-glucosidase)
Aliases:   MG, MGA
RefSeq:   NC_000007.13 NT_007914.15
Ensembl:   ENSG00000257335
Entrez:   8972
Chromosome Mapping:   7q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.920000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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